Profiling primary downstream target of LBD16 in roots of Arabidopsis thaliana

Lateral roots (LRs) are formed post-embryonically and contribute to root architecture formation in vascular plants. LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) is a key transcription factor to initiate LR formation functioning redundantly with related LBD members. To identify primary downstream targets of LBD16, we engineered a transgenic line with inducible LBD16 activity by expressing a fusion protein of LBD16 and rat glucocorticoid receptor (GR) under the regulation of its own regulatory region (gLBD16-GR) in the lbd16-1 lbd18-1 lbd33-1 mutant. Here we identified primary response genes of LBD16 from transcriptome analysis. LBD16-Glucocorticoid Receptor (GR) driven by its own regulatory region in lbd16-1 lbd18-1 lbd33-1 mutant was used for LBD16 induction. We collected roos from 5-day-old seedlings of gLBD16:GR lbd16 lbd18 lbd33 line 4 hours after treatment with/without dexamethazone (DEX) and cycloheximide (CHX). Two independent sets of mRNA samples for each treatment were used for this analysis