Imaging Studies of Local Responses of Prostate Cancer to Radiation Therapy

Patients receiving radiation therapy for prostate cancer received a multiparametric MRI and MRI/ultrasound-fusion targeted biopsy. Patients received a second mpMRI six months after radiation therapy. The goal of this study was to generate gene expression profiles of prostate cancer tumor tissue prior to treatment, and compare the gene expression profiles of those patients based on imaging response or time to biochemical recurrence. FFPE blocks of biopsies targeted to MRI-visible lesions were sectioned onto glass slides, stained with H&E, and macrodissected. Tumor from the same MRI-visible lesion across different blocks were pooled.