Single cell profiling of bone marrow adipocytes and bone marrow stromal cells from irradiated mice.. Single cell profiling of bone marrow adipocytes and bone marrow stromal cells from irradiated mice.

In order to comprehensively characterize bone marrow mesenchymal cells after myeloablation, single-nuclei RNA sequencing was performed on bone marrow adipocytes and bone marrow stromal cells isolated from sublethally-irradiated mice. Overall design: Collected bone marrow adipocytes and sorted CD45/Ter119/CD31- cells from the bone marrow of Plin1-creER; tdTomato mice 5 weeks after sublethal irradiation were kept frozen until nuclei isolation. Cells were resuspended and extracted nuclei for single-nuclei RNA-seq (snRNA-seq).