Lymphoma transcriptome analysis after X-ray or Proton beam irradiation

Proton (PT) therapy represents an alternative to conventional X-ray therapy, and its clinical application for cancer treatment is on the rise due to associated dose deposit advantages. However, our knowledge of biological responses to PT, in comparison to X-ray, remains in its infancy. Identification of PT specific molecular signals is an important opportunity for the discovery of biomarkers and synergistic drugs. We have profiled the transcriptome regulation of lymphoma cells exposed to clinical sources of PT and X-ray radiation, respectively. Subsequent analysis demonstrated both common and radiation-specific deregulation of gene expression. Gene set enrichment discovered pathways unique to PT \ that contribute to the unfolded protein response (UPR) and mitochondrial transport. Expression profiles of BL41 (Human) and J3D (mouse) cell lines untreated or 12hr after exposure to 5Gy X-ray (Linac) or 5Gy Proton beam (Beam scanning, middle SOBP) irradiation