Influence of nuclear proteins on the kinetics of unfolding of the h c-myc GQ structure, assayed by measuring the decrease in FRET activities in the presence of the complementary DNA strand.

*decrease in FRET activity during one minute (% of decrease in the maximal height of the FRET peak measured at 0 time).‡number of experiments.F-myc GQ-R double labeled FRET oligonucleotide (25 pmol) was incubated either alone or with the tested proteins for one minute in a volume of 50 µl, than its FRET spectra was taken (0 min value). The complementary, antiparallel oligonucleotide to the GQ structure (in a tenfold molar excess) was admixed and FRET spectra was recorded at 1, 3 and 5 minutes after annealing has been initiated. The kinetics of the decrease of the FRET peak heights were calculated and shown as ΔFRET peak value during one minute of annealing as percentage of the zero minute FRET peak height values. Excitation was at 485 nm and emissions were recorded between 500–650 nms.