Characteristics of lymphomas in HBZ-Tg and non-Tg littermates.

Mice that died or became immobilized were subjected to autopsy. Tissue samples were surgically removed, fixed in 10% formalin in phosphate buffer, embedded in paraffin and stained with hematoxylin and eosin for histopathological examination. Tissue samples with lymphoma were subjected to immunohistochemical analysis (IHC) using monoclonal antibodies for CD3 (500A2), B220 (RA3-6B2), and Foxp3 (FJK-16s). The phenotype of lymphomas was determined based on CD3 and B220 expression. The degree of Foxp3 expression in lymphomas was evaluated by immunohistochemistry. (+, 1–9%; ++, 10–20%; +++, more than 20%) Frequency of T-cell lymphoma of each line is shown as follows; (number of T-cell lymphoma/number of total observed mice).