Dataset related to article: "Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodies"

This record contains raw data related to article "Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodies" Abstract: Glioblastoma is invariably lethal and responds poorly to immune checkpoint blockade. Here, we examined the impact of regulatory T (Treg) cell depletion on glioblastoma progression and immunotherapy responsiveness. In human glioblastoma, elevated Treg cell signatures correlated with poorer survival outcomes, with these cells expressing high CD25 levels. In Nf1⁻/⁻Pten⁻/⁻EGFRvIII⁺ glioblastoma-bearing mice, a single dose of non-interleukin-2 (IL-2) blocking (NIB) anti-CD25 antibodies depleted Treg cells and promoted CD8⁺ T cell clonal expansion and partial tumor control, further enhanced by PD1-blockade. Treg cell depletion induced interferon-γ-dependent tumor microenvironment remodeling, increasing Fcγ receptor expression on intratumoral myeloid cells and enhancing phagocytosis. Combining anti-CD25ⁿⁱᵇ with anti-EGFRvIII tumor-targeting antibodies resulted in complete tumor control. Anti-human CD25ⁿⁱᵇ treatment of glioblastoma patient-derived tumor fragments effectively depleted Treg cells and activated CD8⁺ T cells. These findings underscore the therapeutic relevance of Treg targeting in glioblastoma and unveil potent combination strategies for anti-CD25ⁿⁱᵇ based on innate cell activation.