Intracellular glucose 6-phosphate and ATP levels and relative increase in serine phosphorylation levels of selected proteins from cultured skeletal muscle cells incubated in the presence or absence of glucose or fructose.

Isolated skeletal muscle cells were incubated for 5 min in the absence or the presence of 10 mM glucose or 10 mM fructose, as described in the Material and Methods. Table shows glucose 6-phosphate and ATP intracellular levels, as well as the relative degree of serine phosphorylation of proteins in which differences above 25% were obtained. Results are expressed in arbitrary units, transforming the intensity obtained to 100 in control points. Different superscripts in a row indicate significant differences following the statistical analysis described in the Material and Methods section. Thus, data marked with an “a” in superscript are significantly different from those marked with any other letter in the same file. Acronyms for the proteins are described in Figure 1. Results are means±S.E.M. for 5 separate experiments.
Intracellular glucose 6-phosphate and ATP levels and relative increase in serine phosphorylation levels of selected proteins from cultured skeletal muscle cells incubated in the presence or absence of glucose or fructose.