Transcriptome Profile of IEC4.1 cells deficient with Prdm1 in response to IFN-gamma

This study is to investigate the potential role of Prdm1 in murine intestinal epithelial cells (IEC4.1 cells) in response to IFN gamma stimulation. Prdm1 was knocked down in IEC4.1 cells by using a CRISPR/Cas9 approach. Wild type IEC4.1 cells were used as control (Ctrl). Cells were treated with or without mouse IFN gamma (1ng/ml for 4hrs). Then total RNA was collected for sequencing via DNBSEQ platform to obtain a comprehensive view of the transcriptome. Overall design: Comparative gene expression profiling analysis of RNA-seq data for ?intestinal epithelial cells (IEC4.1 cells) or IEC4.1_PRDM1 KO cells with or without IFN? treatment.