LHFPL5 is a key element to the gating spring of cochlear hair cells: comparison of mechanotransduction channel activation in presence or absence of LHFPL5

During auditory transduction, sound-evoked vibrations of the hair cell stereociliary bundles open mechanotransducer (MET) ion channels via tip links extending from one stereocilium to its neighbor. How tension in the tip link is delivered to the channel is not fully understood.  The MET channel comprises a pore-forming subunit, transmembrane channel-like protein (TMC1 or TMC2), aided by several accessory proteins, including LHFPL5 (lipoma HMGIC fusion partner-like 5). We investigated the role of LHFPL5 in transduction by comparing MET channel activation in outer hair cells of Lhfpl5-/- knockout mice with those in Lhfpl5+/- heterozygotes. The 10-90 percent working range of transduction in Tmc1+/+; Lhfpl5+/- was 52 nm, from which the single-channel gating force, Z was evaluated as 0.34 pN. However, in Tmc1+/+; Lhfpl5‑/- mice, the working range increased to 123 nm and Z more than halved to 0.13 pN, indicating reduced sensitivity. Tip link tension is thought to activate the channel via a ga..., MET currents were recorded from outer hair cells (OHCs) in isolated organs of Corti of mice between P2 and P7. All whole cell recordings were performed at room temperature, ~23ºC.
All data included in the published article are presented in an Excel file, giving all values obtained to construct the mean +/- 1 SD, for every recorded hair cell in all the different mouse genotypes used in the study.
The Excel file is organized in 3 sheets, describing the MET current bundle displacement results, hair bundle stiffness data, and the hair bundle height measurements.
Stereociliary bundles were stimulated with a fluid jet, and the bundle deflections calibrated by projecting the bundle image onto a pair of photodiodes and measuring the change in photocurrent. The Excel file gives all values obtained for the relationship between the MET current, I, and bundle displacement, X. The I-X relationship was fitted with a Boltzmann equation: I = IMAX /(1 + exp((XO -X)/XS), where IMAX is the maximum current..., , # Data from: LHFPL5 is a key element to the gating spring of cochlear hair cells: comparison of mechanotransduction channel activation properties in presence or absence of LHFPL5

## Description of the data and file structure

Data are readable using Excel software. Data are presented in one single excel file: Lhfpl5_data.xlsx.

Three sheets are present and related to the experimental topic:

**IX Working Range** : Values summarized all data collected for all outer hair cells recordings, used in the published manuscript. WR stands for working range, Imx is the maximum mechanotransduction (MET) current and the age of the animal is given for each cell recorded.

The different colors in the spreadsheet reflect the genotype of the mice recorded. All recording were performed on apical outer hair cells (OHC)

**Stiffness:** Values summarized all data collected for all outer hair cells recordings used in our study. The different colors in the spreadsheet reflect the mice genotype.

Hair bun...