Single cell RNA sequencing of treatment naive Crohn's disease

Crohn's disease (CD) is characterized by chronic inflammation throughout the intestine. Previous single cell transcriptomic studies investigated the effect of CD post therapeutic intervention, however, the extent to which therapy modifies the intestinal inflammatory environment remains to be elucidated. To this end, a treatment naive pediatric CD cohort was recruited to investigate initial disease onset across the intestines, highlighting shared and tissue-specific consequences of disease. A clustering stability assessment workflow was developed to ensure clustering and downstream results were robust. Tensor decomposition was utilized to identify clinically meaningful groups within the intestines.