OMIP-69, Spectral Flow Cytometry, 40 colors, PBMCs of GPA patients and HC

Immune profiling of granulomatosis with polyangiitis (GPA) patients may be useful for tracking disease activity. However, reliable immune signatures for GPA activity are lacking. In this study, we examined circulating immune profiles in GPA patients during active and remission disease states to identify potential immune patterns associated with disease activity. The distribution and phenotypic characteristics of major circulating immune cells were studied on cryopreserved peripheral blood mononuclear cells from GPA patients (active, n=20; remission, n=20) and healthy controls (n=20) leveraging a 40-color optimized multicolor immunofluorescence panel (OMIP-69).
Conclusion:
Deep phenotyping uncovered a distinct composition of major circulating immune cells in active GPA and GPA in remission, with the most significant findings emerging within the monocyte compartment. Our detailed analysis revealed circulating monocyte diversity beyond the conventional monocyte subsets. We identified eight classical monocyte populations, two intermediate monocyte populations, and one non-classical monocyte population. Notably, active GPA had a higher frequency of CD45RA+CCR5+CCR6-CCR7+/lowCD127-HLA-DR+CD2- classical monocytes and a lower frequency of CD45RA-CCR5-/lowCCR6-CCR7-CD127-HLA-DR+CD2+/- classical monocytes, which both strongly correlated with disease activity. Unstained and single-stained cells/beads.