Quantitative proteomic analysis of UUO-14d kidneys from Adamts18+/+ versus Adamts18−/− mice using label-free LC-MS/MS

This project aims to investigate the proteomic differences between Adamts18+/+ and Adamts18−/− mice in response to unilateral ureteral obstruction (UUO) for 14 days. Kidney tissues were collected from both genotypes and subjected to quantitative proteomic analysis using label-free LC-MS/MS-based techniques. The workflow included protein extraction, trypsin digestion, peptide separation, and mass spectrometric analysis. Differentially expressed proteins were identified to explore the molecular mechanisms associated with Adamts18 deficiency in renal fibrosis under UUO conditions.