Effects of Loop Nucleobase Substitution on G-quadruplex Thermal Stability in Aqueous Glycine Betaine, Proline, TMAO, and Urea Solutions

The data included here ascertains the effects of loop nucleobase substitution on G-quadruplex thermal stability in aqueous glycine betaine, proline, TMAO, and urea solutions.

The folder ThermalUnfoldingFiles contains Excel spreadsheets with the raw absorbance versus temperature unfolding data for each G-quadruplex in glycine betaine, proline, TMAO, and urea solutions.

The folder G2 MathematicaTwoStateFitting contains Mathematica notebooks with two-state fits to the data in the ThermalUnfoldingFiles folder.

EstimateG2UnfoldedSurfaceAreaFromdr_SASA.xls is an Excel spreadsheet that calculates the change in solvent accessible surface area of G2 and estimates the m-value/RT in aqueous glycine betaine, proline, TMAO, and urea solutions.

QuadruplexSummary.xlsx is an Excel spreadsheet that consolidates melting temperatures and unfolding enthalpies for the G-quadruplexes to determine m-value/RT values and unfolding enthalpy dependence on cosolute molality in glycine betaine, proline, TMAO, and urea solutions.

G-quadruplex m-value Summary.xslx consolidates m-value/RT values and calculates m-values at melting temperatures in cosolute-free solutions.