Apoptotic MNC-secretomes in experimental stroke

Mixed Model Analysis (SAS output)
The data were analyzed using linear mixed models for the neuroscore on treatment group and time-point with the factor animal included as a random effect. The MIXED procedure in SAS 9.3 was used to perform the calculations. The raw output contains information on the model specifications, the estimated error variance and random effects variance, the estimated regression coefficients, the covariance structure of the model coefficients and type III F-tests for the hypotheses of no effect of either fixed effect or their interactions. An interaction plot was drawn using the GLM procedure. This plot shows the individual observations and their sample mean values in each group and for each time-point. The group labels 0,1,2 and 3 in the raw output refer to the treatment group in setting 1, the control group in setting 1, the treatment group in setting 2 and the control group in setting 2, respectively.
Original Western blots to Figure 5 
Expression of proteins involved in cytoprotective pathways in human Astrocytes and Schwann Cells 
Astrocytes (page 1) or Schwann Cells (page 2) were stimulated with hMNCapo sec, control medium (served as control to treatment) or positive control (control to the measured protein). Original blots for all measured proteins are given in this raw data set (pages 1 and 2). For each blot, lanes (1), (2), and (3) correspond to the groups medium control [(1)=control to treatment], human apoptotic MNC-secretomes [(2)=treatment] and positive control [(3)=recombinant protein].Bands in each blot are shown for phosphorylated CREB, total-CREB, phosphorylated Erk1/2, total-Erk 1/2, phosphorylated HSP27, total-HSP27, phosphorylated cJun, total-cJun, phosphorylated Akt, and total-Akt. The molecular weight (kDa) for each protein can be seen under each blot.Ponceau staining was used as loading control for each group (1), (2), and (3) and suggest equal loading.
Original Western blots to Figure 6 
Expression of Phosphorylated CREB in Astrocytes and Neurons after stimulation with the active compound hMNCapo sec or control medium: 
Cultured human Astrocytes and Neurons were incubated with hMNCapo sec or control (cell culture-) medium at indicated concentrations. Original blots can be seen here.Ponceau staining shows equal loading.

本数据集包含混合模型分析（SAS输出）的相关数据。研究通过对治疗组和时间点的神经评分进行线性混合模型分析，并将动物因素纳入随机效应。计算过程采用了SAS 9.3中的MIXED程序。原始输出中包含了模型规格、估计误差方差和随机效应方差、估计回归系数、模型系数的协方差结构以及固定效应及其交互作用的零效应假设的III型F检验信息。通过GLM程序绘制了交互图，该图展示了每个组别和每个时间点的个体观察值及其样本均值。原始输出中的组别标签0、1、2和3分别对应设置1中的治疗组、设置1中的对照组、设置2中的治疗组和设置2中的对照组。至图5的原始Western印迹分析：涉及细胞保护通路的人类星形胶质细胞和施万细胞的蛋白质表达。星形胶质细胞（第1页）或施万细胞（第2页）被hMNCapo sec、对照培养基（作为治疗的对照）或阳性对照（针对测量蛋白质的对照）刺激。所有测量蛋白质的原始印迹均包含在本原始数据集中（第1页和第2页）。对于每个印迹，泳道（1）、（2）和（3）分别对应于培养基对照组[（1）=治疗对照]、人细胞凋亡MNC分泌组[（2）=治疗]和阳性对照[（3）=重组蛋白]。每个印迹中均显示了磷酸化CREB、总CREB、磷酸化 Erk1/2、总Erk 1/2、磷酸化HSP27、总HSP27、磷酸化cJun、总cJun、磷酸化Akt和总Akt的条带。每个蛋白质的分子量（kDa）均显示在每个印迹下方。Ponceau染色作为每个组别（1）、（2）和（3）的加载对照，提示加载量相等。至图6的原始Western印迹分析：活性化合物hMNCapo sec或对照培养基刺激后星形胶质细胞和神经元中磷酸化CREB的表达：培养的人星形胶质细胞和神经元与hMNCapo sec或对照（细胞培养-）培养基在指示浓度下共孵育。原始印迹在此可见。Ponceau染色显示加载量相等。