RNA sequencing of mice primary microglia from cerebral cortex

Purpose: The differentiation of microglia from M1 to M2 exerts a pivotal role in the aggression of intracerebral hemorrhage (ICH), and long non-coding RNAs (lncRNAs) are associated with the differentiation of microglia. However, the underlying mechanism had not been fully clarified.Methods: The expression profile of lncRNAs in thrombin-induced primary microglia was analyzed by RNA-sequening. Under thrombin treatment, the effect of lncRNA TCONS_00145741 on the differentiation of microglia was determined by immunofluorescence staining, quantitative real-time PCR and Western blot. Besides, the potential mechanism and related signaling pathways of TCONS_00145741 in the M1 and M2 differentiation of microglia in ICH were assessed by Gene Ontology analysis, flow cytometry, RNA pull-down, RNA Immunoprecipitation and RNA fluorescence in situ hybridization followed by immunofluorescence analysis.