Relative expression of mRNA transcripts related to hippocampal plasticity after repeated exposure to extremely-low frequency electromagnetic field (50 Hz)

Methodology:
PCR reactions were performed with LightCycler®96 apparatus by Roche.
Data was acquired from the apparatus and processed with LightCycler® software (Roche, ver. 1.1)
The efficiency of each reaction was calculated with LightCycler® software (Roche, ver. 1.1) with series of dilution of cDNA matrix mix
The quality of the procedures was ensured by usage of triplicates of each sample.
Glyceraldehyde 3-phosphate dehydrogenase was used as a housekeeping gene.

Data-specific information:
Number of variables: 2
Variable list:
1. Electromagnetic field intensity - 1) 1 mT; 2) 7 mT;
2. Number of exposures - 1) one; 2) two; 3) three;

Abbreviations:
E1 - single (seven consecutive days, 1h/day) exposure
E2 - double (2 times of seven consecutive days, 1h/day, with three weeks break in between) exposure
E3 - triple (3 times of seven consecutive days, 1h/day, with three weeks break in between) exposure

C - control group
ELF-EMF/1 mT - group exposed to an extremely-low frequency electromagnetic field of 1 mT
ELF-EMF/7 mT - group exposed to an extremely-low frequency electromagnetic field of 7 mT

Ct - cycle threshold
Bak1 - Bcl-2 homologous antagonist killer
Bcl-2 - B-cell lymphoma 2
Bcl-xL - B-cell lymphoma-extra large
BDNF - brain-derived neurotrophic factor
GAP43 - growth associated protein 43
PSD95 - postsynaptic density protein 95
SYN1 – synapsin I
TrkB - tropomyosin receptor kinase B