Supplemental data for the publication: "Algal methylated compounds shorten the lag phase of Phaeobacter inhibens bacteria"

Data S1: P. inhibens feature table (genes) with results from co-cultivation RNA-sequencing run. The dataset includes bacterial gene accession numbers, functional annotations, transcript abundances (TPM normalized read counts) and results of DESeq2 differential gene expression analysis. (Fig. 1, figs. S1A, S3-S4, tables S1-S2). 

Data S2: P. inhibens feature table (genes) with results from lag phase RNA-sequencing run. The dataset includes bacterial gene accession numbers, functional annotations, transcript abundances (TPM normalized read counts) and results of DESeq2 differential gene expression analysis. (Fig. 4A, figs. S10-S13, tables S6-S7). 

Data S3: Emiliania huxleyi CCMP3266 sGenome gene annotation file version 2 (GFF3 format).

Data S4: Feature quantification obtained using Compound Discoverer. The table presents the output analysis using Compound Discoverer (v3.3) with a putative identification of metabolites. Each identified metabolite (each row) contains a sub-table under the + tab (on the left side) that specifies the feature quantification in each analyzed sample. Sample ID appears in the “Study File ID” column in each sub-table. Values in the columns “Exchange Rate [%]: 0” and “Exchange Rate [%]: 1” represent relative abundances of the molecules in their unlabeled form and with single 13C-label (M+1 isotopologue), respectively. Compounds marked with “1” in the “Tags” column were further validated using standards. The average M+1 isotope abundance [%] for S-Adenosylmethionine (SAM) and 5'-S-Methyl-5'-thioadenosine (MTA)—as reported in Fig. 4D—were calculated by averaging the values in the “Exchange Rate [%]: 1” column from samples supplemented with 13C-labeled and unlabeled DMSP, respectively. Detailed information regarding the isotope abundances of SAM and MTA can be found in the tab “SAM and MTA isotope abundance” in the table. In the tab “Features Positive Mode” samples F10, F12, F14, and F16 were supplemented with 13C-labeled DMSP and samples F2, F4, F6, F8 were supplemented with unlabeled DMSP. In the tab “Features Negative Mode” samples F2, F3, F4, and F5 were supplemented with 13C-labeled DMSP and samples F10, F11, F12, F13 were supplemented with unlabeled DMSP.