An efficient in-gel digestion method on a small amounts of protein sample
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https://www.omicsdi.org/dataset/jpost/PXD024071
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资源简介:
We have verified each step of in-gel digestion. We reduced the sample processing time and labor by optimizing the following four points.
1. Using intact gel pieces.
2. Use 96-well filter and positive pressure manifold before digestion.
3. Extract digested peptide without acetonitrile
4. In order to minimize non-specific absorption, digest in a low-peptides-binding microtube not in the 96-well filter or low-peptides-binding 96-well multiplate.
创建时间:
2023-02-09



