Hypoxia blunts angiogenic signaling and upregulates the antioxidant system in elephant seal endothelial cells

Background
Elephant seals exhibit extreme hypoxemic tolerance derived from repetitive hypoxia/reoxygenation episodes they experience during diving bouts. Real-time assessment of the molecular changes underlying protection against hypoxic injury in seals remains restricted by their at-sea inaccessibility. Hence, we developed a proliferative arterial endothelial cell culture model from elephant seals and used RNA-seq, functional assays, and confocal microscopy to assess the molecular response to prolonged hypoxia.
Results
Seal and human endothelial cells exposed to 1% O2 for up to 6 h respond differently to acute and prolonged hypoxia. Seal cells decouple stabilization of the hypoxia-sensitive transcriptional regulator HIF-1α from angiogenic signaling. Rapid upregulation of genes involved in glutathione (GSH) metabolism supports the maintenance of GSH pools, and intracellular succinate increases in seal but not human cells. High maximal and spare respiratory capacity in seal cells after h..., , , # Data from: Hypoxia blunts angiogenic signaling and upregulates the antioxidant system in elephant seal endothelial cells

[https://doi.org/10.5061/dryad.7pvmcvf0p](https://doi.org/10.5061/dryad.7pvmcvf0p)

Code for RNAseq data associated with \"Hypoxia blunts angiogenic signaling and upregulates the antioxidant system in elephant seal endothelial cells.\"
bioRxiv manuscript doi: 10.1101/2023.07.01.547248
SRA BioProjects: PRJNA1017894 and PRJNA1017895

## Code and files

**config files are separated into seal and human data**
seal_config.json
human_config.json

**compare_config files are separated into seal and human data**
*compare_config files were used to determine differential gene expression at each time point vs control, and pairwise between timepoints using EBSeq*
seal_compare_config.json
human_compare_config.json

**Snakefile** provides snakemake pipeline

**code.R** **contains code for cluster analysis and pathway enrichment for both species, using the following files:**
ma_gene...