Effect of various inhibitors on ATPase activity in gill microsomes from juvenile and adult M. amazonicum.
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Assays were performed continuously at 25°C in 50 mmol L−1 HEPES buffer, pH 7.5, containing 2 mmol L−1 ATP, 5 mmol L−1 MgCl2, 20 mmol L−1 KCl and 50 mmol L−1 NaCl for juveniles or 20 mmol L−1 NaCl for adults, in a final volume of 1.0 mL. Data are the mean ± SD from three (N = 3) different microsomal preparations. Oligomycin was prepared in ethanol. Bafilomycin and thapsigargin were prepared in dimethylsulfoxide.*Significantly different from respective value for juvenile (P≤0.05).
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2015-12-02
