Digging Deeper to Find the Effect of Long-term Greenhouse Cultivation with Excessive Fertilization and Irrigation on the Structure and Assemblage of Soil Bacterial Community

Long-term excessive fertilization and irrigation under greenhouse cultivation systems cause nitrogen leaching, while the residual content varies at different soil depths with cultivation durations. However, it remains unclear whether it changes the composition and assemblage of the soil bacterial community, especially at deeper layers (as deep as 4 m), after long-term intensified cultivation. This study selected soil from three sites in Shouguang (a typical representative intensive planting area), i.e., greenhouse monoculturing for 20 years (G20), greenhouse monoculturing for 10 years (G10), and an adjacent rotation field (F) for physicochemical property determination and high-throughput pyrosequencing.