Hyperuricemia Predisposes to the Onset of Diabetes

To further examine the mechanisms of urate in islet β-cell death, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish primacy of streptozotocin (STZ) vs. urate. Islets were isolated from Uox-KO and WT mice with and without multiple low-dose STZ. Differentially expressed genes (DEGs) in islets of the hyperuricemic and/or diabetic mice in comparison to their respective controls represented specific Gene Ontology (GO) pathways and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional meanings. Diabetic mice models were intraperitoneally injected with multiple low-dose STZ at a dose of 40 mg/kg body weight daily, for 5 consecutive days. Hyperuricemic mice and their counterparts were also included.Four groups (KO, WT, STZ-KO and STZ-WT) were designed with three replications in each.