Microscopy images from: A physiological and histological atlas of reproduction in the North American deer mouse (Peromyscus maniculatus)

The North American deer mouse (Peromyscus maniculatus) exhibits extensive diversity in morphology, physiology, and life history across its broad range. These traits have propelled the deer mouse to model system status across several fields within the biological sciences. Nonetheless, we still lack basic knowledge about some important aspects of this species’ biology. For example, limited information about the deer mouse’s reproductive physiology remains a significant barrier to developing genetic tools for the species and for advancing our current understanding of how this species has been so evolutionarily successful. In this manuscript, we aim to fill this knowledge gap by (1) characterizing body temperature profiles across reproductive stages and (2) generating a detailed histological atlas of placental development. We show that body temperature can be used to diagnose copulation and pregnancy in deer mice, however body temperature cannot be used to predict fertility (likelihood to b..., We employed three separate double-labeling protocols to describe specific features of placentation and development. To examine vascular development and remodeling in the implantation site, we used two sets of sections to label two markers of vascular remodeling and endothelial cell organization: the basement membrane protein laminin and the tight-junction protein ZO-1 (26,27). To examine interactions between uterine natural killer cells (uNK cells) and trophoblasts in the implantation site, we also labeled one set of sections for the uNK cell marker perforin.
For all immunohistochemistry protocols, sections were fixed with ice-cold 4% paraformaldehyde, permeabilized using methanol, and blocked using 10% normal goat serum in PBS (Vector Laboratories S-1000). Sections were incubated overnight with one of three primary antibodies: rabbit anti-laminin (1:1000, Thermo Scientific PA116730), or rabbit anti-ZO-1 (1:500, Invitrogen 617300), or rabbit anti-perforin (1:400, Amsbio TP251). After PB..., , # Microscopy images from: A physiological and histological atlas of reproduction in the North American deer mouse (Peromyscus maniculatus)

[https://doi.org/10.5061/dryad.z34tmpgr7](https://doi.org/10.5061/dryad.z34tmpgr7)

## Description of the data and file structure

These data are raw images of placentas from North American deer mouse,* Peromyscus maniculatus*, implantation sites across gestation. Images were generated using 10 um cryosections of whole implantation sites that were then labeled using immunohistochemistry. Images were captured at varying magnifications with or without stitching on an APX100 microscope. Images contain scale bars.

### Files and variables

#### File: FileMetaData.txt

**Description:** 

##### Variables

* FileName: Full image file name
* ProcessID_Prefix: Process prefix
* LabelingProtocol: Immunohistochemistry labeling regime. Laminin refers to a double-label protocol wherein mCherry (red) indicates laminin, gfp (green) indicates cytokeratin, and DAPI (...,