Flow cytometry data of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice

Data 1. Flow cytometry analysis of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice.Spleen cells were obtained from wild type (A and D), 3H9-transgenic (B and E), and CD40L/3H9 double transgenic mice (C and F). Cells were stained with Alexa Fluor 647-conjugated (FL 8 channel) rat anti-mouse B220 and Pacific Blue-conjugated (FL 6 channel) anti-mouse Ig λ1(LS-136), and with (D-F) or without (A-C) FITC-conjugated (FL 1 channel) goat anti-mouse Ig λ chain. Lymphoid cells were gated by FSC/SSC dot plots and the B220+ cells were analyzed on a CyAn ADP flow cytometer (Beckman Coulter, USA).

数据集1：对野生型、3H9转基因和CD40L/3H9双转基因小鼠的脾细胞进行流式细胞术分析。脾细胞分别来自野生型（A和D）、3H9转基因（B和E）以及CD40L/3H9双转基因小鼠（C和F）。细胞经Alexa Fluor 647偶联（FL 8通道）的鼠抗小鼠B220和Pacific Blue偶联（FL 6通道）的鼠抗小鼠Ig λ1（LS-136）染色，并在（D-F）组或（A-C）组中，使用FITC偶联（FL 1通道）的羊抗小鼠Ig λ链进行染色。通过FSC/SSC点图对淋巴样细胞进行门控，并在CyAn ADP流式细胞仪（Beckman Coulter，美国）上对B220阳性细胞进行分析。