A pan-family screen of Nuclear Receptors in immunocytes reveals ligand-dependent inflammasome control [scRNA-seq]

To examine more closely the varied effects of RA receptor deletions on peritoneal MFs, and determine whether inactivation of RXRa and RAR? elicited superimposable effects on different LPM clusters (as predicted if they operate as a heterodimer, a known propensity of RXR proteins), we performed single-cell RNA sequencing (scRNAseq) analyses on total CD11b+CD115+ peritoneal cells from BMC mice edited for several RA receptors, 17 days after reconstitution. Overall design: Total CD11b+CD115+ peritoneal macrophages were sorted from BMC mice carrying RA receptor mutants (i.e., RORa, RXRß ,RAR? and RXRa) and internal controls, 17 days after reconstitution. Each in biological duplicate, and all multiplexed by hashtagging into the same run for optimal comparability.