Analysis of chromatin accessibility upon NODAL/Activin signalling reveal mechanisms of SMAD2-regulated transcription

In this experiment patterns of chromatin accessibility were compared in P19 mouse embryonic teratoma cells treated with either the Nodal/Activin singalling inhibitor SB-431542, or with Activin for 1hr (acute signalling) and 8 hr (prolonged signalling), or left untreated (chronic signalling) in this study we performed ATAC-seq in P19 mouse embryonic teratoma cells in four different signaling states: inhibited (SB-431542 treated); acute (1 hr Activin-treated); prolonged (8 hr Activin-treated) and untreated (chronic signalling). Experments were done in biological duplicates.