Supplementary Data for Hua et al. (2020)

This dataset contains supplementary data for the analyses presented in:<br><br>Zhengshuang Hua, Matthew Ouellette, Andrea M. Makkay, R. Thane Papke, and Olga Zhaxybayeva. <i>Nutrient supplementation experiments on saltern microbial communities support utilization of DNA as a source of phosphorus</i> (under review).<br><br><b>Dataset contents:<br></b><br><b>Quality_Control_script.zip:</b> In-house Perl script used for quality control processing of raw Illumina reads. See README.md file inside the archive for details.<br><br><b>merged_pairs.zip:</b> Merged pairs from the rpoB and 16S rRNA data after the quality control step, removal of primers, and additional removal of low quality sequences. The sequences are in FASTA format.<br><br><b>rpoB_database.zip:</b> Curated database of 627 rpoB gene sequences used for taxonomic assignment of a representative sequence of an rpoB-based OTU. <i>Halobacteria.rpoB.627.fa</i> file contains nucleotide sequences in FASTA format. <i>Halobacteria.rpoB.627.taxonomy</i> file contains the taxonomic information for each sequence.<br><br><b>OTU_tables_and_rep_seqs.zip</b>: OTU tables for rpoB- and 16S rRNA-based OTUs (<i>otu_table.rpoB.txt</i> and <i>otu_table.16S.txt</i> files). Each row represents an OTU. Each column represent a sample. Values in the table show how many assembled contigs are in an OTU. Nucleotide sequences of chosen to represent each OTU are provided in rep_set_rpoB.fna and rep_set_16S.fna files, in FASTA format.<br><br>