Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq]

We demonstrate that actin interacts with essentially all transcribed genes and co-occupies most gene promoters together with RNA polymerase II (Pol II). On highly expressed genes, actin and Pol II can be found also on the gene bodies. Overall design: chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) of Mrtf-GFP, actin (AC-74 and AC-15) and Pol II phosphorylated at serine 5 (Pol II S5P) in ovaries of wild type (w1118) and Mrtf mutant (mal-d?7) flies, where Mrtf expression is abolished in ovaries, as well as in flies ubiquitously expressing GFP-tagged version of Mrtf (tub mal-d3xGFP)