Gene expression profiling from pooled samples of subcutaneous adipose tissue of NAPE-WT or NAPE-KO mice fed either with a control diet or a high-fat diet

Mice knocked-out or wild type for the NAPE PLD gene specifically in adipose tissue, were recruited for this expression profiling experiment. Each group of mice (WT versus cKO) were fed with a control diet or a high fat diet. Then mice were sacrificed and adipose tissue samples form the subcutaneous adipose tissue were processed for RNA extraction. Total RNA of each sample was then pooled with those of the same group and treatment for microarray hybridization. two groups of mice: (I) NAPE PLD WT and NAPE PLD KO. Each group was submitted to a diet treatment: (I) control diet and (II) high fat diet.本表达谱分析实验招募了脂肪组织特异性敲除NAPE PLD基因的小鼠与野生型（Wild Type, WT）小鼠。实验将小鼠分为两组：(I) NAPE PLD野生型组与(II) NAPE PLD敲除组，每组均接受两种饮食处理：对照饲料与(II) 高脂饲料。随后处死小鼠，采集皮下脂肪组织样本进行RNA提取，将同组且同处理的各样本的总RNA混合后用于基因芯片杂交。