Bromodomain inhibition decreases virulence in a mouse model of infection.

(A) Growth curves for two different strains used in the study (L224 and SM). I-BET151-treated cells were incubated at 37°C and counted daily on a hemacytometer and parasite numbers were compared with control cells. * indicates a t-test p-value of RAG-/- mice infected with trypanosomes pretreated in vitro with I-BET151 or DMSO (vehicle control) for the indicated number of days. * indicates significant p-value of p-value of RAG-/- mouse was sacrificed at day ten due to infection unrelated to trypanosomiasis. (D) Survival curves of C57/B6 mice infected with Bdf3kd trypanosomes and fed with Dox pellets for 2 d prior to infection (Pre Dox), or once parasitemia reached detectable levels (Post Dox) compared with mice fed with regular pellets. p-Value derived from Log-rank Mantel-Cox test performed on the three groups. (E) Peak levels of parasites/ml of blood measured by tail bleed in Dox-fed wildtype mice infected with Bdf2KO trypanosomes compared to mice fed with regular pellets. p-Value derived from Mann-Whitney test. Numerical data for Fig 6 is in S6 Data.