Transcriptome wide identification of retained introns upon depletion of the splicing factors WBP11 or SNW1. Transcriptome wide identification of retained introns upon depletion of the splicing factors WBP11 or SNW1

We identified introns that are dependent on WBP11 or SNW1 for efficient splicing. WBP11 and SNW1 were depleted by RNAi and compared to a mock transfected population of cells. WBP11 was also depleted by auxin and compared to an untreated population of cells Overall design: RNA-Seq analysis of mRNA purified from colorectal adenocarcinoma cell lines depleted of either WBP11 or SNW1 by RNAi or by auxin-induced depletion of AID-tagged WBP11 transgene in an endogenous WBP11 knockout background