Nano-ImmunoEra. WP2 T2.1. ECL from beads on lasered SPCE

This folder stores data related to Task 2.1 ECL from beads on lasered SPCE within Work Package 2 Production Characterization Materials for Improved ECL-based Biosensing, in the NanoimmunoEra Horizon 2020 project. The data includes ECL (Electrochemiluminescence) images and quantitative ECL results about 2.8 micrometer streptavidin-coated magnetic beads. We reported here an innovative approach to enhancing the ECL properties of screen-printed carbon electrodes (SPCEs) through post-fabrication CO2 laser treatment. By examining three different carbon paste-based electrodes with advanced ECL microscopy, we demonstrate that laser treatment at low energy doses (7–12 mJ/cm²) effectively improves the electrochemical properties of the material by removing surface binders and impurities. Quantitative analysis with a photomultiplier tube (PMT) revealed that GST electrodes can sensitively detect biomarkers with a limit of detection (LOD) of approximately 11 antibodies/µm². These findings underscore the potential of laser-treated GST electrodes for high-sensitivity ECL immunoassays and biosensing applications.