Population RNA-seq and ATAC-seq of SLAMF6+ and CXCR6+ Th17 cells

To characterize and validate the functional roles of the splenic SLAMF6+ and CXCR6+ Th17 cells during EAE, we performed population RNA-seq and ATAC-seq for wild type mice, as well as population RNA-seq for Il17aCre Il23rfl/fl knockout mice. We isolated SLAMF6+ and CXCR6+ Th17 cells from the spleen during EAE for three independent experiments: (1) RNA-seq for cells from four Il17aCre tdTomato reporter mice; (2) ATAC-seq for cells from five Il17aCre tdTomato reporter mice. (3) RNA-seq for cells from four Il17aCre Il23rfl/fl (Il23r KO) vs. four Il17aCre Il23rWT (Il23r WT) mice.