scRNA-seq of human PCLS

Precision-cut lung slices (PCLS) are increasingly utilized for ex-vivo disease modeling, but a high-resolution characterization of cellular-phenotype stability in PCLS has not been reported. Comparing the single cell transcriptomic profile of human PCLS after five days of culture to freshly isolated human lung tissue, we found striking changes in  endothelial cells and alveolar epithelial cell programs, reflecting both injury and pathways activated in static culture, while  immune cell frequencies and programs remained largely intact and similar to the native lung. These baseline changes should be considered when utilizing PCLS as a model of the human lung. Examination of scRNA-seq of human precision-cut lung slices after 5 days in culture