ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes

ETV1 (ER81) was identified as a highly enriched transcription factor in rapidly conducting atrial and His-Purkinje cardiomyocytes. Previous work has shown that ETV1 is a critical factor in determining fast conduction physiology in the heart. This high-throughput sequencing project aims to uncover ETV1-dependent gene networks in cardiomyocytes. These results provide insight into the role of ETV1 in the establishment of rapid conduction zones in the heart. Neonatal rat ventricular myocytes were isolated and purified from postnatal day 1 Sprague-Dawley wild type male and female rats using Miltenyi Biotec rat neonatal cardiomyocyte isolation kit (Miltenyi Biotec, 130-105-420) according to the manufacture's protocol. The final myocyte cultures contained enriched cardiomyocytes at a density of 3X—10^6 cells per 35-mm dish. Myocytes cultures were transduced with either Ad-ETV1-GFP or Ad-GFP 24 hours after plating. RNA was extracted at day 4 post-transduction to analyze transcriptional differences with the overexpression of ETV1 in cardiomyocytes.