The influence of post-glacial migration and hybridization on the gene pool of marginal Quercus pubescens populations in Central Europe

Background and Aims In Central Europe, the drought-tolerant downy oak (Quercus pubescens) is at the northern edge of its natural distribution range, often growing in small and spatially isolated populations. Here, we elucidate how the population genetic structure of Central European Q. pubescens was shaped by geographic barriers, genetic drift and introgression with the closely related sessile oak (Q. petraea).
Methods 27 Q. pubescens populations from the northern margin of Q. pubescens’ natural distribution range were sampled. Based on 16 nuclear microsatellite markers (nSSRs), Bayesian clustering and distance-based analyses were performed to determine the intraspecific genetic structure and to identify genetic barriers. To identify drivers of introgression with Q. petraea, generalised linear models were applied to link levels of introgression with environmental conditions. To track post-glacial migration routes, the spatial distribution of haplotypes based on 8 chloroplast microsatell..., Genotyping of nSSRs and cpSSRs: To determine the length of the amplified fragments (alleles) in base pairs (bp), capillary electrophoresis was performed using the sequencer SeqStudio Genetic Analyzer from Applied Biosystems by Thermo Fisher Scientific. The software GeneMapper® Software 6 (Applied Biosystems) was used to perform peak scoring. Exported allele lists have been stored as text files. 
Georeferenced samples: All samples have been georeferenced in the field by using a GPS device. All coordinates were stored in decimal degree (WGS 84)., , # Data from: The influence of post-glacial migration and hybridization on the gene pool of marginal Quercus pubescens populations in Central Europe

[https://doi.org/10.5061/dryad.vhhmgqp4d](https://doi.org/10.5061/dryad.vhhmgqp4d)

## Description of the data and file structure

Genotyping: All individuals were genotyped with nSSRs (nuclear microsatellite markers), whereas cpSSRs (chloroplast microsatellite markers) were amplified for every second individual of a population. To determine the length of the amplified fragments (alleles) in base pairs (bp), capillary electrophoresis was performed using the sequencer SeqStudio Genetic Analyzer from Applied Biosystems by Thermo Fisher Scientific. The software GeneMapper® Software 6 (Applied Biosystems) was used to perform peak scoring.

Georeferencing of individuals: All individuals were georeferenced in the field by using a GPS device. Geographic positions are stored in decimal degree (WGS 84)

### Files and variables

#### File: DRYAD\_Dat...