RNA-seq of floe1-1 vs Col-0 seeds

We identified a regulator of Arabidopsis thaliana seed germination: FLOE1 (AT4G28300). We used RNA-seq to uncover genes that are diffenrentially regulated in dry seeds, imbibed seeds and seeds imbibed in 220mM NaCl. Imbibition and salt: after 5 days of stratification at 4C in the dark on MS media containing either 0 or 220mM NaCl, seeds were transferred to a growth chamber at 22C with normal light conditions for 4hours. RNA was then extracted from these seeds as well as from dry seeds. RNA was then sent to Novogene Corporation Inc. for library preparation (250-300 bp insert cDNA library) and sequencing (2x150 bp paired-end reads on an Illumina Platform). Three replicates were conducted for each genotype (floe1-1 or Col-0) and condition (dry, imbibed, salt).