通过特定位点扩增片段测序对华北落叶松进行遗传定位

A high-density genetic linkage map is essential for plant genetics and genomics research. However, due to the deficiency of genomic data and high-quality molecular markers, no genetic map has been published for Prince Rupprecht’s larch (Larix principis-rupprechtiiMayr), a conifer species with high ecological and commercial value in northern China. In this study , 145 F1 progeny individuals from an intraspecific cross between two elite clones of L. principis-rupprechtii and their parents were employed to construct the first genetic map in this important trees peciesusing specific-locusamplified fragment sequencing (SLAF-seq).