Data from Aquaref 2024 project, metabarcoding for the Diatoms Biological Index, France

These data were generated as part of the Aquaref 2024 project for the assessment of the Diatom Biological Index (DBI) required by the Water Framework Directive (WFD). Samples were collected by french Water Agencies and OFB laboratories (Adour-Garonne, Rhin-Meuse, Loire-Bretagne, Rhône-Méditerannée-Corse, Seine-Normandie, Artois-Picardie). DNA was extracted using the NucleoMag® DNA Microbiome kit (Macherey-Nagel). DNA was amplified by PCR using RbcL primers (3FW + 2RV). FastQ files were obtain after DNA sequencing based on the Illumina MiSeq technology with the v3 reagent kit (2 x 250pb) at the GeT-Biopuces platform (Toulouse, France). This directory contains the following files : Aquaref_2024_Run1.tar.gz : all samples FastQ files from the first run 2024 (368 x 2 replicates) Aquaref_2024_Run2.tar.gz : all samples FastQ files from the second run 2024 (57 x 2 replicates) Metadata_Aquaref_2024.xlsx : contains all the data available for each samples corresponding to the names of FastQ files. Metadata_Aquaref_2024.csv : contains all the data available for each samples corresponding to the names of FastQ files.