RNA-Seq profiling in two chicken cell lines, LMH and DF1 with different susceptibiltiy to H9N2 avian influenza virus

We utilize the natural cell line model (LMH and DF1) with different susceptibiltiy to H9N2 avian influenza virus to find out more and new potential key factors of influencing AIV infection and replication via a high-throughput RNA sequencing (RNA-seq). DF1 and LMH cells were infected with H9N2 subtype HN strain at 0.1 MOI.Viral replication waw detected at 4, 6, 12, 24, 36 and 48 hours post infection (h. p. i).Total RNA were respectively extracted from DF1 or LMH cells infected with HN strain at 6 h. p. i and 12 h. p. i using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturer’s protocol. For every time point, each sample was collected in duplication. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and checked using RNase free agarose gel electrophoresis. The samples were used for RNA-Seq