IL-15 Complex-Stimulated NK cells Protect Mice from Cerebral Malaria

We report a comparison of purified mouse NK cells from mice treated in vivo with either IL-2 cytokine complexes or IL-15 cytokine complexes. We isolated RNA and performed HiSeq 2500 analysis on paired end reads of 125 base pairs in triplicate. We find relatively few significant gene expression changes between the groups, but discovered that IL-10 was induced with IL-15 cytokine complex treatment. We show that NK produced IL-10 rescues mice from lethal cerebral malaria. Overall design: Sort purified splenic NK cells (NK1.1+NKp46+CD3-CD1d tetramer-) from female C57BL6 mice treated with either IL-2 cytokine complexes or IL-15 cytokine complexes