RNA-Seq of Cellulophaga baltica and its phage phi14:2 during the infection process

We performed high-througput RNA sequencing of RNA samples extracted from C. baltica cells infected with phi14:2 phage at 40, 90, 140 and 190 minutes after infection. The same experiments were performed with cells treated with host RNA polymerase inhibitor in order to determine which genes rely on viral rifampicin-insensitive RNA polymerase. Using this data, we identified different time classes of phage genes and revealed genes transcribed by phage RNA polymerase. Overall design: The differences of transcript abundances of phage genes at different stages of infection were analyzed. The ratios of transcript abundances between rifampicin-treated and untreated samples were calculated.