Recovery of the full in vivo firing range in post-lesion surviving DA SN neurons associated with Kv4.3-mediated pacemaker plasticity

Dopamine (DA) neurons in the substantia nigra (SN) control several essential functions, including the voluntary movement, learning and motivated behavior. Healthy DA SN neurons show diverse firing patterns in vivo, ranging from slow pacemaker-like activity (1-10 Hz) to transient high frequency bursts (<100 Hz), interspersed with pauses that can last hundreds of milliseconds. Recent in vivo patch experiments have started to reveal the subthreshold mechanisms underlying this physiological diversity, but the impact of challenges like cell loss on the in vivo activity of adult DA SN neurons, and how these may relate to behavioral disturbances, are still largely unknown. We investigated the in vivoelectrophysiological properties of surviving SN DA neurons after partial unilateral 6-OHDA lesions, a single-hit, non-progressive model of neuronal cell loss. We show that mice subjected to this model have an initial motor impairment, measured by asymmetrical rotations in the open field test, wh...,
Animals
Male and female C57Bl/6N mice (Charles River Laboratories) were used for the study. The mice were 8 weeks old, group housed and maintained on a 12-hour light-dark cycle. All experiments and procedures involving mice were approved by the German Regierungspräsidium Darmstadt (V54-19c20/15-F40/30). In total, 152 mice were used for this study (see table below).

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Early phase
Late phase

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Mice (#)
Cells (#)
Mice (#)
Cells (#)

In vivo juxta & behavior – vehicle

15
12 – medial/ 28 – all
12
10 – medial/ 26 – all

In vivo juxta & behavior – 6-OHDA

16
9 – medial/ 16 – all
10
9 – medial/ 11 – all

In vitro – vehicle

3
16
3
12

In vitro – 6-OHDA

6
37
5
25

In vitro & AmmTx3 – vehicle

-
-
3
15

In vitro & AmmTx3 – 6-OHDA

-
-
3
14

In vitro voltage clamp experiments – vehicle

-
-
3
17

In vitro voltage clamp experiments – 6-OHDA

-
 
3
15

Female behavior experiments – vehicle, 6-OHDA
-
 
10
 

Female ..., # Recovery of the full in vivo firing range in post-lesion surviving DA SN neurons associated with Kv4.3-mediated pacemaker plasticity

## GENERAL INFORMATION

Date of data collection: 2021–2024

## OVERVIEW

This dataset provides raw and processed electrophysiological recordings and immunohistochemistry data supporting the results of the study investigating intrinsic plasticity of surviving substantia nigra (SN) dopamine neurons following partial 6-OHDA lesion. The focus lies on the temporal evolution of firing adaptations and Kv4.3-mediated homeostatic plasticity.

## FILE STRUCTURE AND CONTENT

```
/physiology_data.zip/
│
├── /in_vitro/
│ ├── /6OHDA_early/
│ │ ├── CChyp.zip
│ │ ├── contCC.zip
│ │ └── onCell.zip
│ ├── /6OHDA_late/
│ │ ├── CChyp.zip
│ │ ├── contCC.zip
│ │ └── onCell.zip
│ ├── /6OHDA_late_AmmTx3/
│ │ ├── CChyp.zip
│ │ ├── contCC.zip
│ │ └── onCell.zip
│ ├── /6OHDA_late_voltageClamp/
│ │ ├── Activation.zip
│ │ ├── HCN.z...,