CODEX multiplexed imaging cell datasets used for using STELLAR to transfer cell type annotations to other tissues and donors
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https://datadryad.org/dataset/doi:10.5061/dryad.g4f4qrfrc
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资源简介:
We performed CODEX (co-detection by indexing) multiplexed imaging
on 24 sections of the human intestine from 3 donors (B004, B005, B006)
using a panel of 47 oligonucleotide-barcoded antibodies. We also
performed CODEX imaging on both human tonsil and Barrett's
esophagus (BE) using a panel of 57 oligonucleotide-barcoded
antibodies. Subsequently images underwent standard CODEX image
processing (tile stitching, drift compensation, cycle concatenation,
background subtraction, deconvolution, and determination of best focal
plane), single cell segmentation, and column marker z-normalization by
tissue. Output of this process were dataframes of 870,000 cells and
220,000 cells respectively with fluorescence values quantified from each
marker.
提供机构:
Dryad
创建时间:
2022-07-19



