RNAseq of RPE-hTert cells expressing different levels of predictive reporter construct

RNAseq of different subpopulations of RPE1 cells expressing predictive reporter construct, pre- and on Day 6 post-induction with 4OHT Parent ('N') RPE1-hTert cells were infected with the predictive reporter (NLS-mVenus-P2A-ER:HRAS^G12V) construct then flow sorted into subpopulations based on increasing mVenus intensity ('S' < 'M' < 'L' < 'XL'). Cells were harvested for RNA extraction pre- and on Day 6 post-induction with 4OHT. Five biological replicates of each sample were sequenced.