Replicated transcriptome profiling of Normal and Cancerous Prostate Cells [RNA-Seq]. Homo sapiens

To identify differentially expressed transcripts in Cancer and Normal Prostate cells global transcript abundance was assayed by replicated (n=3) stranded RNA-Sequencing Overall design: PolyA+ RNA profiles of LNCaP (n=3) and PrEC (n=3) cell lines were generated by deep sequencing. Libraries were generated by Illumina TruSeq kits (maintaining strand information). The resultant cDNA libraries were sequenced at the Kinghorn Center for Clinical Genomics (The Garvan Institute of Medical Research, Darlinghurst, NSW, Australia) using the Illumina HiSeq 2500 platform.