DNA sequencing for single nucleotide polymorphisms in opioid receptor (OPRM1) and Catechol-O-methyltransferase (COMT)

DNA sequencing for single nucleotide polymorphisms in opioid receptor (OPRM1) and Catechol-O-methyltransferase (COMT). The MiSeq System (Illumina), with a 2 x 78bp read length, was used in sequencing the OPRM1, and COMT gene. The proprietary system TargetRich was used to capture specific genome regions that were relevant to pharmacogenetics, according the Kailos Genetics Inc. protocol. Once sequenced, Kailos blue, the cloud-based analysis system, performed: (i) sample demultiplexing, (ii) quality assessment, (iii) alignment to the genome, (iv) variant calling, and (v) report generation.