Bone metastatic variant of MCF7 cells

A bone-metastatic variant of MCF7 cells was created from a spontaneous metastasis from the mammary fat pad to the bone marrow in the absence of exogenous estrogen. The goal of the study was to determine differences in gene expression between the parental MCF7 cells and the bone metastatic variant. Overall design: There are three biological replicates of the control MCF7 cells containing a non-silencing shRNA construct, which was used in the initial experiment to create the bone-metastatic variant, and three biological replicates of the MCF7 bone metastatic cells (MCF7b). Each biological replicate was collected from independent experiments.