Expression of apoptotic proteins in mice expressing VWF/pV1316M.

For each experiment, 12 mice were used: 2 injected with pLIVE, 4 injected with WT and 6 injected with VWF/p.V1316M mice. Washed murine platelets (2.5 x108 platelets/mL) were lysed and then an equal number of platelets was loaded. Apoptotic proteins were assessed by immunoblotting with anti-Bak, anti-Bax and anti-14-3-3ζ antibodies. Data are expressed as the ratio of apoptotic protein expression versus 14-3-3ζ expression. Then, the ratio for mVWF/p.V1316M or WT mVWF was compared with the corresponding ratio for control pLIVE plasmid (100%) and results are presented as means ± SEM from three independent experiments.